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Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17
Author(s) -
Chung WeiJen,
Shu HungYu,
Lu ChiYu,
Wu ChiuYi,
Tseng YiHsiung,
Tsai ShihFeng,
Lin ChaoHsiung
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600647
Subject(s) - proteome , xanthomonas campestris , kegg , biology , xanthomonas campestris pv. campestris , virulence , pathovar , proteomics , genome , microbiology and biotechnology , bacteria , genetics , gene , transcriptome , gene expression , pseudomonas , pseudomonadaceae
The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2‐DE and the well‐resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence‐related proteins. We detected 22 up‐regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti‐host defense protein, Ohr. Thus, further functional studies of these up‐regulated proteins with respect to their roles in XCC pathogenicity are suggested.