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Ruthenium (II) tris‐bathophenanthroline disulfonate is well suitable for Tris‐Glycine PAGE but not for Bis‐Tris gels
Author(s) -
Moebius Jan,
Denker Katrin,
Sickmann Albert
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600642
Subject(s) - tris , ruthenium , hydroxymethyl , chemistry , fluorescence , chromatography , glycine , organic chemistry , biochemistry , amino acid , physics , quantum mechanics , catalysis
Pre‐cast bis(2‐hydroxyethyl)iminotris(hydroxymethyl)methane (Bis‐Tris) gels have proven to be very suitable for pre‐fractionation for LC‐MS/MS analysis due to high reliability and long stability. To visualize proteins within gels fluorescence dyes proved to be a good tradeoff between sensitivity and MS‐compatibility. The custom‐made ruthenium dye represents a low‐cost alternative regarding fluorescence‐based protein visualization with high sensitivity. We demonstrate, that this dye is incompatible with Bis‐Tris gels, while using Tris‐Glycine gels a competitive sensitivity to commercially available stains can be achieved.