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Proteomic analysis of vascular endothelial cells in response to laminar shear stress
Author(s) -
Wang XiaoLi,
Fu Alex,
Raghavakaimal Sreekumar,
Lee HonChi
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600568
Subject(s) - western blot , proteome , shear stress , integrin , proteomics , signal transduction , microbiology and biotechnology , biology , receptor , chemistry , biochemistry , materials science , gene , composite material
Isotope‐coded affinity tags (cICAT) coupled with mass spectrometric analysis is one of the leading technologies for quantitative proteomic profiling and protein quantification. We performed proteomic analysis of bovine aortic endothelial cells (BAEC) in response to laminar shear stress using cICAT labeling coupled with LC‐MS/MS. Protein expressions in BAEC under 15 dynes/cm 2 of shear stress for 10 min, 3 h, and 6 h were compared with matched stationary controls. Analysis of each sample produced 1800–2400 proteins at ≥75% confidence level. We found 142, 213, and 186 candidate proteins that were up‐ or down‐regulated by at least two‐fold after 10 min, 3 h, and 6 h of shear stress, respectively. Some of these proteins have known cellular functions and they encompass many signaling pathways. The signaling pathways that respond to shear stress include those of integrins, G‐protein‐coupled receptors, glutamate receptors, PI3K/AKT, apoptosis, Notch and cAMP‐mediated signaling pathways. The validity of the mass spectrometric analysis was also confirmed by Western blot and confocal immunofluorescence microscopy. The present quantitative proteomic analysis suggests novel potential regulatory mechanisms in vascular endothelial cells in response to shear stress. These results provide preliminary footprints for further studies on the signaling mechanisms induced by shear stress.