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Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2‐DE of acidic proteins
Author(s) -
Delaplace Pierre,
van der Wal Froukje,
Dierick JeanFrançois,
Cordewener Jan H. G.,
Fauconnier MarieLaure,
du Jardin Patrick,
America Antoine H. P.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600493
Subject(s) - chromatography , extraction (chemistry) , proteomics , chemistry , solid phase extraction , lysis buffer , phenol extraction , protein purification , lysis , resolution (logic) , biochemistry , computer science , rna , artificial intelligence , gene
Two protein extraction procedures were tested in order to remove interfering compounds prior to 2‐DE of potato tubers. These methods using SDS lysis buffer and phenol‐phase extraction were compared regarding the quality of the resulting 2‐D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the M r range of preferentially extracted proteins.