Premium
Lipid raft proteome of the human neutrophil azurophil granule
Author(s) -
FeukLagerstedt Elisabeth,
Movitz Charlotta,
Pellmé Sara,
Dahlgren Claes,
Karlsson Anna
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600482
Subject(s) - azurophilic granule , lipid raft , microbiology and biotechnology , granule (geology) , membrane protein , cytoskeleton , chemistry , proteome , membrane , biology , biochemistry , cell , inflammation , immunology , myeloperoxidase , paleontology
Detergent‐resistant membrane domains (DRMs) are present in the membranes of azurophil granules in human neutrophils (Feuk‐Lagerstedt et al., J. Leukoc. Biol. 2002, 72 , 970). Using a proteomic approach, we have now identified 106 proteins in a DRM preparation from these granule membranes. Among these proteins were the lipid raft structural proteins flotillin‐1 and ‐2, cytoskeletal proteins such as actin, vimentin and tubulin, and membrane fusion promoting proteins like annexins and dysferlin. Our results suggest that the azurophil granule membrane, in similarity to the plasma membrane, is an elaborate structure that takes part in intracellular signaling and functions other than the mere delivery of bactericidal effector molecules to the phagosome.