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Proteomic analysis of bacterial‐blight defense‐responsive proteins in rice leaf blades
Author(s) -
Mahmood Tariq,
Jan Asad,
Kakishima Makoto,
Komatsu Setsuko
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600470
Subject(s) - biology , rubisco , plant defense against herbivory , jasmonic acid , thaumatin , defence mechanisms , pathogenesis related protein , xanthomonas oryzae , proteomics , pseudomonas syringae , inoculation , microbiology and biotechnology , chaperonin , pathogen , botany , biochemistry , gene , gene expression , horticulture
Abstract Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo7435) and incompatible (T7174) races of Xanthomonas oryzae pv. oryzae ( Xoo ). Cytosolic and membrane proteins were fractionated from the leaf blades and separated by 2‐D PAGE. From 366 proteins analyzed, 20 were differentially expressed in response to bacterial inoculation. These proteins were categorized into classes related to energy (30%), metabolism (20%), and defense (20%). Among the 20 proteins, ribulose‐1,5‐bisphosphate carboxylase/oxygenase large subunit (RuBisCO LSU) was fragmented into two smaller proteins by T7174 and Xo7435 inoculation. Treatment with jasmonic acid (JA), a signaling molecule in plant defense responses, changed the level of protein accumulation for 5 of the 20 proteins. Thaumatin‐like protein and probenazole‐inducible protein (PBZ) were commonly up‐regulated by T7174 and Xo7435 inoculation and JA treatment. These results suggest that synthesis of the defense‐related thaumatin‐like protein and PBZ are stimulated by JA in the defense response pathway of rice against bacterial blight.