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AFM fishing nanotechnology is the way to reverse the Avogadro number in proteomics
Author(s) -
Archakov Alexander Ivanovich,
Ivanov Yurii Dmitrievich,
Lisitsa Andrey Valerevich,
Zgoda Victor Gavrilovich
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600467
Subject(s) - avogadro constant , nanotechnology , atomic force microscopy , proteomics , biomolecule , chemistry , materials science , physics , biochemistry , gene , thermodynamics
Future development of proteomics may be hindered by limitations in the concentration sensitivity of widespread technological approaches. The concentration sensitivity limit (CSL) of currently used approaches, like 2‐DE/LC separation coupled with MS detection, etc., varies from 10 −9 to 10 −12 M. Therefore, proteomic technologies enable detection of up to 20% of the protein species present in the plasma. New technologies, like atomic force microscopy (AFM molecular detector), enable the counting of single molecules, whereas biospecific fishing can be used to capture these molecules from the biomaterial. At the same time, fishing also has thermodynamic limitations due to the reversibility of the binding. In cases where the fishing becomes irreversible, its combination with an AFM detector enables the registration of single protein molecules, and that opens up a way to lower the CSL down to the reverse Avogadro number.