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Proteome profiles of mucosal immunoglobulin uptake in inflamed porcine gut
Author(s) -
Danielsen Marianne,
Thymann Thomas,
Jensen Bent Borg,
Jensen Ole Nørregaard,
Sangild Per Torp,
Bendixen Emøke
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600463
Subject(s) - proteome , colostrum , antibody , biology , immunology , immune system , inflammation , immunity , immunoglobulin a , endocytosis , immunoglobulin g , microbiology and biotechnology , receptor , biochemistry
Acquisition of passive immunity by endocytosis of intact immunoglobulins (Ig) from colostrum is critical for prevention of intestinal and systemic diseases in neonatal mammals. We compared proteome patterns of healthy and inflamed gut tissues from pre‐term piglets to investigate the effect of inflammation on acquisition of passive immunity. A clear difference in the two‐dimensional gel electrophoresis protein patterns between healthy and inflamed intestinal tissues was observed, suggesting that inflamed tissues failed to absorb and transfer Ig from colostrum to epithelial cells. We have mapped and identified the Ig proteins that are taken up by healthy intestinal tissues, and found that isoforms of the IgA and IgG heavy chain and Ig kappa and lambda light chains were internalized. Our results indicate that colostrum protein uptake in the porcine gut is a selective process that is obstructed in inflamed pre‐term gut.