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High‐throughput analysis of mumps virus and the virus‐specific monoclonal antibody on the arrays of a cationic polyelectrolyte with a spectral SPR biosensor
Author(s) -
Kim HyunSoo,
Jung SeHui,
Kim SangHyun,
Suh InBum,
Kim Woo Jin,
Jung JaeWan,
Yuk Jong seol,
Kim YoungMyeong,
Ha KwonSoo
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600432
Subject(s) - polyelectrolyte , surface plasmon resonance , biosensor , adsorption , mumps virus , virus , monolayer , chemistry , materials science , nanotechnology , virology , nanoparticle , biology , polymer , organic chemistry
We investigated the potential use of a spectral surface plasmon resonance (SPR) biosensor in a high‐throughput analysis of mumps virus and a mumps virus‐specific mAb on the arrays of a cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA). The PDDA surface was constructed by electrostatic adsorption of the polyelectrolyte onto a monolayer of 11‐mercaptoundecanoic acid (MUA). Poly‐ L ‐lysine was also adsorbed onto the MUA monolayer and compared with the PDDA surface in the capacity of mumps virus immobilization. The PDDA surface showed a higher adsorption of mumps virus than the poly‐ L ‐lysine surface. The SPR signal caused by the virus binding onto the PDDA surface was proportional to the concentration of mumps virus from 0.5 × 10 5 to 14 × 10 5 pfu/mL. The surface structure of the virus arrays was visualized by atomic force microscopy. Then, a dose‐dependent increase in the SPR signal was observed when various concentrations of the antimumps virus antibody in buffer or human serum were applied to the virus arrays, and their interaction was specific. Thus, it is likely that the spectral SPR biosensor based on the cationic polyelectrolyte surface may provide an efficient system for a high‐throughput analysis of intact virus and serodiagnosis of infectious diseases.