Premium
Selective enrichment of Ser‐/Thr‐phosphorylated peptides in the presence of Ser‐/Thr‐glycosylated peptides
Author(s) -
Poot Alex J.,
Ruijter Eelco,
Nuijens Timo,
Dirksen Eef H. C.,
Heck Albert J. R.,
Slijper Monique,
Rijkers Dirk T. S.,
Liskamp Rob M. J.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600373
Subject(s) - threonine , serine , chemistry , phosphorylation , peptide , biochemistry , glycosylation
Modification through β‐elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine‐phopshorylated (Ser‐/Thr) peptides. However, under harsh basic conditions, Ser‐/Thr‐glycosylated peptides are susceptible to β‐elimination as well. Therefore, we have optimized these conditions to achieve a β‐elimination that is highly selective for phosphorylated peptides. This is the first report of selective β‐elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides.