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Autoantigen arrays for multiplex analysis of antibody isotypes
Author(s) -
Graham Kareem L.,
Vaysberg Maria,
Kuo Annie,
Utz Paul J.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600345
Subject(s) - isotype , multiplex , antigen , antibody , biology , monoclonal antibody , immunology , microarray , microbiology and biotechnology , computational biology , genetics , gene , gene expression
We describe here a microarray‐based method for multiplexed, antigen‐specific assessment of immunoglobulin (Ig) subclasses. We used 1152‐feature arrays composed of 140 antigens or antigen fragments to detect isotype‐specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen‐specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.