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Proteomic profiles of induced hepatotoxicity at the subcellular level
Author(s) -
Zgoda Victor,
Tikhonova Olga,
Viglinskaya Anastasia,
Serebriakova Marina,
Lisitsa Andrey,
Archakov Alexander
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600342
Subject(s) - cytosol , microsome , proteome , chemistry , ultracentrifuge , proteomics , phenobarbital , biochemistry , biology , enzyme , pharmacology , gene
In the present study proteomes of liver samples were analyzed after administration of phenobarbital (PB) or 3‐methylcholantrene (3‐MC) to mice. Liver cell homogenates were subfractionated by differential ultracentrifugation into cytosol and microsomes, which were subjected to 2‐DE to generate the proteomic maps of these fractions. 2‐DE yielded 1100 and 800 protein spots for microsomes and cytosol, respectively. General trends of the fraction‐specific alterations after 3‐MC or PB treatment were evaluated using the Student's t ‐test and the principal component analysis (PCA). According to the PCA‐derived data, the microsomal changes after 3‐MC and PB treatment were quite similar. However, in the case of the cytosol data, the specificities of 3‐MC‐ and PB‐induced responses could be clearly distinguished from each other. Protein spots, whose expression levels differed from control, were identified by MALDI‐TOF PMF. Proteomic studies such as those reported herein can be useful in identifying the molecular‐based toxicity of lead drug candidates.