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Lipopolysaccharide‐stimulated responses in rat aortic endothelial cells by a systems biology approach
Author(s) -
Tseng HsiangWen,
Juan HsuehFen,
Huang HsuanCheng,
Lin John YiChung,
Sinchaikul Supachok,
Lai TziChung,
Chen ChiehFu,
Chen ShuiTein,
Wang GueiJane
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600296
Subject(s) - inflammation , biology , signal transduction , microbiology and biotechnology , microarray analysis techniques , kegg , immune system , lipopolysaccharide , microarray , cytokine , tumor necrosis factor alpha , immunology , gene , gene expression , transcriptome , genetics
The vascular endothelium plays an important role in regulating immune and inflammatory responses to resist pathogens infection. Although it has been known that lipopolysaccharide (LPS) is a critical inducer of sepsis or endotoxemia, the systematic responses of LPS‐stimulation in endothelial cells (ECs) are still unclear. The present study aims to analyze the late‐phase responses of LPS‐induced rat aortic ECs by using systematic biology approaches, including rat cDNA microarray, 2‐DE and MALDI‐TOF MS/MS, and cytokine protein array. Furthermore, to improve the efficiency of analysis of the bulk systematic data of rat, we designed a set of bioinformatic tools to convert and integrate these rat data into the corresponding human genes or proteins IDs based on BioCarta, KEGG, and Gene Ontology databases. Using the systematic analysis, it was shown that LPS could promote some signaling or metabolic pathways as well as pathophysiologic phenomena of proliferation, atherogenesis, inflammation, and apoptosis through activated nuclear factor‐κB pathway in ECs. Interestingly, ECs also activated the mediators of anti‐inflammation, antiapoptosis, and antioxidation to protect themselves. Moreover, the expressions of altered genes, proteins, and their involvement in the hypothetical signaling pathway can provide further understanding of inflammation associated responses in ECs.