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Preparation of monoclonal antibody bank against whole water‐soluble proteins from rapid‐growing bamboo shoots
Author(s) -
Wu YuJen,
Chen HanMin,
Wu TaiTse,
Wu JiannShing,
Chu ReaMin,
Juang RongHuay
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600278
Subject(s) - monoclonal antibody , proteome , antibody , biology , shoot , bamboo , fusion protein , immunostaining , botany , chemistry , biochemistry , microbiology and biotechnology , immunohistochemistry , immunology , gene , recombinant dna
An antibody bank against the whole proteins in a proteome is a useful tool for biological research. Using the standard cell fusion method, and a modified screening protocol, we produced an mAb bank against the total water‐soluble proteins extracted from the rapid‐growing green bamboo shoots. An improved two‐stage strategy was employed to enrich those poor immunogenic or lower expressed proteins. Totally, we obtained a bank of 192 mAb which were identified as distinctive to each other by 2‐DE and immunostaining.