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Identification of proteins of potential diagnostic value for bovine paratuberculosis
Author(s) -
Cho Donghee,
Sung Nackmoon,
Collins Michael T.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600207
Subject(s) - paratuberculosis , identification (biology) , computational biology , value (mathematics) , biology , chemistry , computer science , genetics , mycobacterium , bacteria , botany , machine learning
Previously we showed that Mycobacterium paratuberculosis culture filtrates (CFs) contain more antigens that react with sera from infected cattle than do cellular extracts of the organism. The goal of the present study was to identify proteins of potential diagnostic value among these CF proteins. Proteins of potential interest were first separated by 2‐DE. Roughly 240 CF protein spots were detected on CBB‐stained gels using Phoretix 2D software. Of these, 83% reacted with serum from M. paratuberculosis ‐infected cattle in immunoblots. When bovine serum was absorbed with M. phlei antigens, however, only 37 of these antigenic protein spots were reactive. Twenty‐four of these spots were selected for identification based on their immunoblot staining intensity and differences in p I and mass. A total of 14 proteins were ultimately identified by MS and BLAST searches as ModD, PepA, ArgJ, CobT, Antigen 85C, and nine hypothetical proteins. N‐terminal peptide analysis of PepA, Antigen 85C, ModD, MAP1693c, MAP2168c, and MAP1022c showed that each protein has 27–39 amino acids that may function as a signal sequence suggesting they are secreted through a Sec‐dependent pathway. These 14 proteins from M. paratuberculosis CF are strong candidates for use as antigens for improved serodiagnostic tests for bovine paratuberculosis.