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Proteomic analysis of mouse growth plate cartilage
Author(s) -
Belluoccio Daniele,
Wilson Richard,
Thornton David J.,
Wallis Tristan P.,
Gorman Jeffrey J.,
Bateman John F.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600191
Subject(s) - cartilage , extracellular matrix , blot , microbiology and biotechnology , proteomics , matrix (chemical analysis) , biology , chemistry , anatomy , biochemistry , gene , chromatography
Cartilage is a highly specialized load‐bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2‐DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2‐DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.