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Detection and identification of bacterial cell surface proteins by fluorescent labeling
Author(s) -
Anaya Cecilia,
Church Nadia,
Lewis Janina P.
Publication year - 2007
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600175
Subject(s) - bacteria , porphyromonas gingivalis , bacterial cell structure , fluorescence , cell , cyanine , biology , surface protein , biochemistry , chemistry , microbiology and biotechnology , genetics , physics , quantum mechanics , virology
A rapid method of detection and identification of bacterial cell surface proteins is needed to better understand the interaction of bacteria with host components. To detect cell surface proteins, we have labeled cells of the Gram‐negative anaerobic bacterium, Porphyromonas gingivalis , with fluorescent cyanine dyes, Cy3 and Cy5. We demonstrate that only cell surface proteins were labeled, indicating the method applied in our study is suitable for detection and identification of cell surface proteins in Gram‐negative bacteria and possibly other organisms.