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CyDye Immunoblotting for Proteomics: Co‐detection of specific immunoreactive and total protein profiles
Author(s) -
Donoghue Pamela M.,
McManus Ciara A.,
O'Donoghue Niaobh M.,
Pennington Stephen R.,
Dunn Michael J.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600139
Subject(s) - proteomics , blot , quantitative proteomics , antibody , microbiology and biotechnology , chemistry , biology , biochemistry , immunology , gene
Abstract The development of ECL‐Plex CyDye‐conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre‐labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2‐D immunoprobing and protein confirmation following MS analysis.