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Improved protocol for chromatofocusing on the ProteomeLab PF2D
Author(s) -
Barré Olivier,
Solioz Marc
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600074
Subject(s) - chromatofocusing , protocol (science) , computational biology , biology , computer science , medicine , biochemistry , pathology , alternative medicine , enzyme , isoelectric point
Beckman‐Coulter has recently introduced the ProteomeLab PF2D™ for 2‐D liquid separation of protein samples. The system features separation in the first dimension by chromatofocusing, followed by RP chromatography in the second dimension, allowing the analysis of complex proteomics samples. When used by the standard protocol, reproducibility and column life times are limited, making the use of the instrument very costly. We here present an improved protocol for chromatofocusing, which enhances column life by at least fivefold.