Premium
Searching cell‐secreted proteomes for potential urinary bladder tumor markers
Author(s) -
Lin ChiaoYun,
Tsui KeHung,
Yu ChihChia,
Yeh ChunWei,
Chang PheiLang,
Yung Benjamin YatMing
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600066
Subject(s) - proteome , urine , western blot , bladder cancer , cancer research , blot , urinary system , tumor marker , plasminogen activator , carcinoma , cell culture , cancer , cell , biology , urinary bladder , medicine , pathology , bioinformatics , gene , biochemistry , genetics
To search for biomarkers critical for bladder carcinoma diagnosis and prognosis, secreted proteomes of highly malignant U1 and pre‐malignant U4 cell lines were initially analyzed. Proteins in the culture media of the U1 and U4 cell lines were systematically examined by SDS‐PAGE combined with MALDI‐TOF MS. Among them, expression of pro‐u‐plasminogen activator (pro‐u‐PA) was confirmed by Western blot analysis and further evaluated. In analyzing urine samples from bladder cancer patients and normal subjects, we established a statistically significant relationship between the low level and absence of pro‐u‐PA in urine with high stages and grades of the tumor samples. Constitutive expression of Ras dominant negative protein led to increased expression of pro‐u‐PA in culture media, indicating that the loss of pro‐u‐PA is associated with oncogenic transformation. Analysis of cancer‐secreted proteomes can be a feasible, non‐invasive and efficient strategy for searching potential bladder tumor biomarkers. Our work also has identified the loss of pro‐u‐PA in urine as potential marker of more advanced bladder carcinoma.