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Protein expression of lymphocytes in HLA‐DR transgenic pigs by a proteomic approach
Author(s) -
Huang SanYuan,
Chen YuHui,
Teng ShihHua,
Chen IChung,
Ho LinLin,
Tu ChingFu
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600054
Subject(s) - biology , transgene , microbiology and biotechnology , proteomics , human leukocyte antigen , lymphocyte , function (biology) , genetically modified mouse , gene , antigen , immunology , genetics
Matching donor and recipient human leucocyte antigen (HLA‐II) could conquer cell‐mediated rejection following transplantation. Transgenic pigs carrying HLA genes that “humanize” porcine organs, tissues, and cells were successfully generated. This study further clarifies the effect of HLA‐DR transgenes on lymphocyte protein expression, via a proteomic approach. Lymphocytes were isolated from two HLA‐DR transgenic pigs and three nontransgenic littermates on 157 d after birth. Soluble protein of 1×10 7 cells was separated using 2‐DE. In total, 301 colloidal CBB‐stained protein spots detected on all five 2‐D gels were quantified. Thirty‐three proteins were differentially expressed by a factor of 1.5. These proteins were subsequently identified by MALDI‐TOF MS and MALDI‐TOF/TOF MS/MS. These proteins were sorted into the following categories: chaperones, T‐lymphocyte function, DNA/RNA processing, cytoskeleton‐associated proteins, signal transduction, enzymes, and unknown. Previous studies have suggested that some of the identified proteins are associated with lymphocyte activation/proliferation. The identities of the unidentified spots and the systematic effect of these up‐ and down‐regulated proteins on T‐cell function in HLA‐DR transgenic pigs require further exploration.