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Shotgun proteomic analysis of human‐induced sputum
Author(s) -
Nicholas Ben,
Skipp Paul,
Mould Richard,
Rennard Stephen,
Davies Donna E.,
O'Connor C. David,
Djukanović Ratko
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200600011
Subject(s) - proteome , sputum , proteomics , biology , shotgun proteomics , computational biology , mucin , human proteome project , human disease , bioinformatics , genetics , biochemistry , medicine , pathology , gene , tuberculosis
Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin‐rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human‐induced sputum (sputome). A combination of 2‐D gel analysis and GeLC‐MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.