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Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane
Author(s) -
Tanaka Masayoshi,
Okamura Yoshiko,
Arakaki Atsushi,
Tanaka Tsuyoshi,
Takeyama Haruko,
Matsunaga Tadashi
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500887
Subject(s) - magnetosome , magnetotactic bacteria , organelle , transmembrane protein , biogenesis , cytoplasm , biology , membrane protein , lipid bilayer , bacteria , microbiology and biotechnology , membrane , biochemistry , chemistry , gene , genetics , receptor
Prokaryotes are known to have evolved one or more unique organelles. Although several hypotheses have been proposed concerning the biogenesis of these intracellular components, the majority of these proposals remains unclear. Magnetotactic bacteria synthesize intracellular magnetosomes that are enclosed by lipid bilayer membranes. From the identification and characterization of several surface and transmembrane magnetosome proteins, we have postulated that magnetosomes are derived from the cytoplasmic membrane (CM). To confirm this hypothesis, a comparative proteomic analysis of the magnetosome membrane (MM) and CM of the magnetotactic bacterium, Magnetospirillum magneticum AMB‐1, was undertaken. Based on the whole genome sequence of M. magneticum AMB‐1, 78 identified MM proteins were also found to be prevalent in the CM, several of which are related to magnetosome biosynthesis, such as Mms13, which is tightly bound on the magnetite surface. Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM.