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A proteomic study on human osteoblastic cells proliferation and differentiation
Author(s) -
Spreafico Adriano,
Frediani Bruno,
Capperucci Caterina,
Chellini Francesca,
Paffetti Alessandro,
D'Ambrosio Chiara,
Bernardini Giulia,
Mini Roberta,
Collodel Giulia,
Scaloni Andrea,
Marcolongo Roberto,
Santucci Annalisa
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500858
Subject(s) - osteosarcoma , osteoblast , cell growth , cell culture , microbiology and biotechnology , cellular differentiation , biology , proteomics , cell , human bone , cancer research , in vitro , biochemistry , gene , genetics
Changes in expression profiles for 17 proteins were ascertained in human mature osteoblasts compared to pre‐osteoblasts (differentiation markers). A differential approach was used to highlight proteomic changes between human osteosarcoma cells and mature osteoblasts, showing a relative over‐expression of 8 proteins (proliferation and tumor indicators), as well as under‐expression of proteins also found down‐regulated in pre‐osteoblasts (specific markers of osteoblast differentiation). Our findings confirmed the differences between cell lines and primary human cell cultures and suggested caution on the use of osteosarcoma to study anti‐osteoporotic drugs in humans.