Premium
Proteomic analysis of pulmonary sclerosing hemangioma
Author(s) -
Jin LianJin,
Shin Bong Kyung,
Jung Woon Yong,
Lee HyunJuu,
Cho Su Jin,
Han JoungHo,
Ha SeongYeon,
Kim AeRee,
Sik Kim Young,
Sun Kim In,
Uhm ChangSub,
Kim Han Kyeom
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500819
Subject(s) - cytokeratin , immunohistochemistry , western blot , microbiology and biotechnology , pathology , biology , vimentin , pathogenesis , hemangioma , blot , medicine , biochemistry , gene
Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been clear. Surfactant protein, thyroid transcription factor‐1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2‐D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI‐TOF‐MS, and database searching. Apolipoprotein A‐1, antizyme inhibitor, heat shock 27‐kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down‐regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.