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Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid
Author(s) -
Kim YongSoo,
Kim MyungSun,
Lee SookHwan,
Choi BumChae,
Lim JeongMook,
Cha Kwang Yul,
Baek KwangHyun
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500775
Subject(s) - follicular fluid , fibrinogen , follicular phase , andrology , antithrombin , biology , population , western blot , oocyte , endocrinology , medicine , biochemistry , microbiology and biotechnology , heparin , embryo , gene , environmental health
Recurrent spontaneous abortion (RSA), defined as the loss of three or more consecutive pregnancies prior to the 20th week of gestation, affects up to 5% of the child‐bearing population. To investigate the proteins associated with RSA, the protein expression in human follicular fluid was analyzed using 2‐DE. Follicular fluid contains a variety of biologically important proteins for oocyte fertilization and follicle maturation in the mammalian reproductive process. Therefore, it can be used as a provisional source for identifying proteins involved in RSA. In this study, we identified five aberrantly expressed proteins (complement component C3c chain E, fibrinogen γ, antithrombin, angiotensinogen, and hemopexin precursor) in follicular fluid from RSA patients with MALDI‐TOF‐MS and nano‐LC MS/MS. Western blot analysis confirmed that the protein expression level of fibrinogen γ and antithrombin was less in follicular fluid from RSA patients than those from normal controls. Semiquantitative RT‐PCR and real‐time PCR analyses revealed that mRNA level of these coagulation factors was also decreased significantly in chorionic villi of RSA patients compared with normal samples. Taken all together, it is likely that coagulation factors (fibrinogen γ and antithrombin) play an important role in maintaining the normal pregnancy.