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Self‐assembling protein arrays on DNA chips by auto‐labeling fusion proteins with a single DNA address
Author(s) -
Jongsma Maarten A.,
Litjens Ralph H. G. M.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500654
Subject(s) - dna , oligonucleotide , fusion protein , recombinant dna , lysis , chemistry , microbiology and biotechnology , biochemistry , biology , gene
The high‐throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self‐assembly concepts. DNA‐directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O 6 ‐alkylguanine‐DNA‐alkyltransferase (SNAP‐tag™) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.