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Shotgun identification of proteins from uredospores of the bean rust Uromyces appendiculatus
Author(s) -
Cooper Bret,
Garrett Wesley M.,
Campbell Kimberly B.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500630
Subject(s) - shotgun , shotgun proteomics , identification (biology) , computational biology , biology , proteomics , botany , biochemistry , gene
We are interested in learning more about the proteome of Uromyces appendiculatus , the fungus that causes common bean rust. Knowledge of the proteins that differentiate life‐cycle stages and distinguish infectious bodies such as uredospores, germlings, appressoria, and haustoria may be used to define host–pathogen interactions or serve as targets for chemical inhibition of the fungus. We have used 2‐D nanoflowLC‐MS/MS to identify more than 400 proteins from asexual uredospores. A majority of the proteins appear to have roles in protein folding or protein catabolism. We present a model by which an abundance of heat shock proteins and translation elongation factors may enhance a spore's ability to survive environmental stresses and rapidly initiate protein production upon germination.