A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate‐polyacrylamide electrophoresis gels | Zendy

Jin LiTai | Zendy; Hwang SunYoung | Zendy; Yoo GyurngSoo | Zendy; Choi JungKap | Zendy

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A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate‐polyacrylamide electrophoresis gels

Author(s) -

Jin LiTai,

Hwang SunYoung,

Yoo GyurngSoo,

Choi JungKap

Publication year - 2006

Publication title -

proteomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.26

H-Index - 167

eISSN - 1615-9861

pISSN - 1615-9853

DOI - 10.1002/pmic.200500596

Subject(s) - silver stain , chemistry , sodium dodecyl sulfate , mass spectrometry , chromatography , eriochrome black t , staining , sodium , silver nitrate , polyacrylamide gel electrophoresis , polyacrylamide , gel electrophoresis , electrophoresis , nuclear chemistry , biochemistry , microbiology and biotechnology , polymer chemistry , adsorption , enzyme , organic chemistry , biology , medicine , pathology

A quick, sensitive, and MALDI‐TOF MS compatible silver staining method, namely Eriochrome black T (EBT)‐silver method, is described. The method can detect 0.05–0.2 ng protein within 60 min in SDS‐PAGE gels. EBT dye was used as a silver ion sensitizer having reducing power for silver ions.

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