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A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate‐polyacrylamide electrophoresis gels
Author(s) -
Jin LiTai,
Hwang SunYoung,
Yoo GyurngSoo,
Choi JungKap
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500596
Subject(s) - silver stain , chemistry , sodium dodecyl sulfate , mass spectrometry , chromatography , eriochrome black t , staining , sodium , silver nitrate , polyacrylamide gel electrophoresis , polyacrylamide , gel electrophoresis , electrophoresis , nuclear chemistry , biochemistry , microbiology and biotechnology , polymer chemistry , adsorption , enzyme , organic chemistry , biology , medicine , pathology
A quick, sensitive, and MALDI‐TOF MS compatible silver staining method, namely Eriochrome black T (EBT)‐silver method, is described. The method can detect 0.05–0.2 ng protein within 60 min in SDS‐PAGE gels. EBT dye was used as a silver ion sensitizer having reducing power for silver ions.