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Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections
Author(s) -
Bernal Dolores,
Carpena Inés,
Espert Ana Maria,
De la Rubia José Enrique,
Esteban José Guillermo,
Toledo Rafael,
Marcilla Antonio
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500571
Subject(s) - aldolase a , biology , glyceraldehyde 3 phosphate dehydrogenase , tropomyosin , proteome , enolase , heat shock protein , hsp70 , excretory system , heterologous , proteomics , phosphoglycerate mutase , actin , cytoskeleton , phosphoglycerate kinase , fructose bisphosphate aldolase , two dimensional gel electrophoresis , enzyme , biochemistry , glycolysis , dehydrogenase , immunology , immunohistochemistry , cell , gene , endocrinology
In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma –rodent model, suggesting that these proteins may play a role in the survival within the host.
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