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Is protein overlap in two‐dimensional gels a serious practical problem?
Author(s) -
Hunsucker Stephen W.,
Duncan Mark W.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500521
Subject(s) - proteomics , proteome , protein expression , quantitative proteomics , posttranslational modification , data science , computer science , computational biology , key (lock) , biochemical engineering , chemistry , biology , bioinformatics , biochemistry , engineering , enzyme , computer security , gene
In a recently published article, Campostrini et al. [ Proteomics 2005, 5 , 2385–2395] raised questions regarding the utility of 2‐D gels in proteomics research. We believe that the authors have overlooked several key issues including the dynamic range of protein expression and the sensitivity of the analytical methods used to explore a proteome. We argue that 2‐D gels have and will continue to provide meaningful quantitative data when applied to proteomic analysis and that the practical significance of spot overlap has been overstated.