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Ligand profiling and identification technology for searching bioactive ligands
Author(s) -
Baek MoonChang,
Kim SunJin,
Yea Kyungmoo,
Kim Youndong,
Lee ByungDae,
Kim Jaeyoon,
Lee HaeJeong,
Kang MeeHee,
Choi SunKyu,
Kim JongIn,
Lee Taehoon G.,
Suh PannGhill,
Ryu Sung Ho
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500511
Subject(s) - profiling (computer programming) , chemistry , peptide , computational biology , chromatography , combinatorial chemistry , affinity chromatography , small molecule , biology , computer science , biochemistry , enzyme , operating system
We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano‐LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low‐abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.