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A functional proteomics approach for the detection of nuclear proteins based on derepressed importin alpha
Author(s) -
Blazek Erik,
Meisterernst Michael
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500449
Subject(s) - proteomics , proteome , computational biology , importin , biology , identification (biology) , nuclear protein , alpha (finance) , sequence (biology) , nuclear transport , cell nucleus , microbiology and biotechnology , bioinformatics , biochemistry , nucleus , gene , medicine , construct validity , botany , nursing , transcription factor , patient satisfaction
Abstract The identification of functional proteomes is a major challenge in proteomic research. Here we describe a method for the detection and isolation of nuclear (localization sequence containing) proteins using a derepressed import receptor (DIRE) as a synthetic antibody. We demonstrate that the DIRE method specifically detects nuclear localization sequence containing proteins. Application to activation of primary T‐lymphocytes exemplifies the potential use of DIRE for comparative proteomics and for diagnostics.