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Proteotyping of human haptoglobin by MALDI‐TOF profiling: Phenotype distribution in a population of toxic oil syndrome patients
Author(s) -
Rodriguez Carlos,
Quero Carmen,
Dominguez Ana,
Trigo Miguel,
Posada de la Paz Manuel,
Gelpi Emilio,
Abian Joaquin
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500434
Subject(s) - haptoglobin , phenotype , profiling (computer programming) , biology , population , proteomics , computational biology , medicine , genetics , immunology , gene , environmental health , computer science , operating system
Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981 as a consequence of the ingestion of an aniline‐adulterated oil illegally marketed as edible. TOS affected more than 20 000 people and produced over 400 deaths in the first 18 months after the outbreak. There is evidence that genetic factors could play a role in the susceptibility of individuals towards the disease. Recently, we suggested that haptoglobin (Hp) polymorphism could also play a role in TOS. To provide a rapid method for high‐throughput Hp phenotyping, we developed a two‐step MALDI‐TOF procedure that allows specific identification of the three common Hp α chains. Resolution of the homologous α‐1s and α‐1f chains, which have a mass difference of only 0.043 Da, is obtained after guanidination of the protein with O ‐methylisourea. We applied this procedure to the study of the distribution of the Hp alleles HP 1s , HP 1f , HP 2 in a control versus a TOS‐affected population, both originally exposed to the toxic oil. The MALDI‐TOF proteotyping method was validated by a parallel analysis of the serum samples by 2‐DE. Data obtained from 54 TOS cases and 48 control individuals indicate significant differences in the distribution of Hp phenotypes in the two populations.