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Proteomic analysis of the tegument and excretory‐secretory products of adult Schistosoma bovis worms
Author(s) -
PérezSánchez Ricardo,
RamajoHernández Alicia,
RamajoMartín Vicente,
Oleaga Ana
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500420
Subject(s) - viral tegument , parasite hosting , biology , host (biology) , proteomics , schistosoma , antigen , secretory protein , proteome , pathogen , lysozyme , excretory system , microbiology and biotechnology , schistosoma mansoni , helminths , virology , immunology , schistosomiasis , biochemistry , secretion , genetics , anatomy , gene , world wide web , computer science
Schistosoma bovis is a ruminant pathogen that is poorly known at a molecular level. With an aim of identifying the parasite proteins involved in host‐parasite interplay, we studied two protein extracts that contain, respectively, the proteins excreted/secreted by the adult worm (ES) and the tegumental proteins exposed to the host (TG). The 2‐DE, 2‐D immunoblot and MS were employed to separate and identify the antigenic proteins and the most abundant non‐antigenic proteins in each extract. There were some 400 and 600 spots detected in the ES and the TG extracts, respectively. Ninety‐six spots were subjected to MS analysis and 64 of them were identified. Overall, we identified 18 S. bovis proteins located at the host‐parasite interface, 16 of which have not been identified previously in this parasite, and one of which ‐lysozyme‐ has never been reported in a Schistosoma species. Of the proteins identified, at least 4 can counteract host defence mechanisms. The other proteins are also likely to play some role in the host‐parasite relationships. Therefore, studies in grater depth on all these proteins will provide a better understanding of how this parasite interacts with its host and new strategies for anti‐schistosome drug or vaccine design.