Premium
Identification of target proteins of N ‐acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration
Author(s) -
Kim YongSam,
Kang HyeYeon,
Kim JinYoung,
Oh Sejeong,
Kim CheorlHo,
Ryu Chun Jeih,
Miyoshi Eiji,
Taniguchi Naoyuki,
Ko Jeong Heon
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500400
Subject(s) - colorectal cancer , protein tyrosine phosphatase , western blot , cancer , cancer research , glycosylation , biology , microbiology and biotechnology , tyrosine , biochemistry , gene , genetics
To gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2‐DE and lectin blot, followed by identification based on ESI‐MS. Through these approaches 14 proteins were identified as candidate target proteins for N ‐acetylglucosaminyl transferase V (GnT‐V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPκ) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPκ underwent an aberrant glycosylation in GnT‐V‐overexpressing WiDr cells, and the aberrantly glycosylated PTPκ was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPκ in GnT‐V‐overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.