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A proteomic approach to study pea ( Pisum sativum ) responses to powdery mildew ( Erysiphe pisi )
Author(s) -
Curto Miguel,
Camafeita Emilio,
Lopez Juan A.,
Maldonado Ana M.,
Rubiales Diego,
Jorrín Jesús V.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500396
Subject(s) - powdery mildew , spots , inoculation , biology , sativum , pisum , proteome , botany , horticulture , biochemistry
As a global approach to gain a better understanding of the mechanisms involved in pea resistance to Erysiphe pisi , changes in the leaf proteome of two pea genotypes differing in their resistance phenotype were analyzed by a combination of 2‐DE and MALDI‐TOF/TOF MS. Leaf proteins from control non‐inoculated and inoculated susceptible (Messire) and resistant (JI2480) plants were resolved by 2‐DE, with IEF in the 5–8 pH range and SDS‐PAGE on 12% gels. CBB‐stained gels revealed the existence of quantitative and qualitative differences between extracts from: (i) non‐inoculated leaves of both genotypes (77 spots); (ii) inoculated and non‐inoculated Messire leaves (19 spots); and (iii) inoculated and non‐inoculated JI2480 leaves (12 spots). Some of the differential spots have been identified, after MALDI‐TOF/TOF analysis and database searching, as proteins belonging to several functional categories, including photosynthesis and carbon metabolism, energy production, stress and defense, protein synthesis and degradation and signal transduction. Results are discussed in terms of constitutive and induced elements involved in pea resistance against Erysiphe pisi .