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Profiling of differential protein expression in angiogenin‐induced HUVECs using antibody‐arrayed ProteoChip
Author(s) -
Ahn EunHee,
Kang DongKu,
Chang SooIk,
Kang Chang Soo,
Han MoonHi,
Kang InCheol
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500394
Subject(s) - angiogenin , antibody microarray , western blot , proteome , dna microarray , protein microarray , biology , proteomics , microbiology and biotechnology , polyclonal antibodies , antibody , microarray , protein array analysis , gene expression , angiogenesis , bioinformatics , immunology , biochemistry , genetics , gene
Abstract ProteoChip has been developed as a novel protein microarray technology. So far it has been applied in new lead screening and molecular diagnosticsand we expect its role to grow in the field of biology. Here, we investigated the application of ProteoChip for the study of differential protein expression profiles in angiogenin‐induced human umbilical vein endothelial cells (HUVECs). Antibody microarrays constructed by immobilizing 60 distinct antibodies against signal‐transducing proteins on ProteoChip base plates were used to analyze the expression pattern of cell‐signaling proteins in HUVECs treated with angiogenin. The antibody microarray approach showed that angiogenin induced the up‐ and down‐regulation of several cellular regulators related with cell proliferation. Changes in the expression of signaling proteins determined by antibody microarray were validated by Western blot analysis. In this experiment, ten up‐regulated proteins and six down‐regulated proteins were identified and confirmed by immunoblot analysis. Taken together, these data suggest that antibody microarrays using ProteoChip technology can be a powerful tool for high‐throughput analysis of proteomes in biological samples.