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Protein nanoarray on Prolinker™ surface constructed by atomic force microscopy dip‐pen nanolithography for analysis of protein interaction
Author(s) -
Lee Minsu,
Kang DongKu,
Yang HyunKyu,
Park KeunHyung,
Choe Soo Young,
Kang ChangSoo,
Chang SooIk,
Han Moon Hi,
Kang InCheol
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500392
Subject(s) - nanotechnology , nanolithography , materials science , atomic force microscopy , contact angle , chemistry , fabrication , medicine , alternative medicine , pathology , composite material
Protein nanoarrays are addressable ensembles of nano‐scale protein domain on solid surfaces. This method can serve as a useful platform for ultraminiaturized bioanalysis. In this study, we investigated single molecular nanopatterning and molecular interaction of proteins that were immobilized on Prolinker™ surface of gold‐coated silicon wafer by using dip‐pen nanolithography (DPN) method. Contact force and humidity were optimized at 0.01 nN and 80%, respectively. The domain features of protein nanoarrays were developed at the contact time of 5 s. The optimized conditions for the nanoarray process were applied to create protein nanoarray using integrin α v β 3 and angiogenin. Constructed protein nanoarrays using integrin α v β 3 have single molecular monolayer with regular domain shape (height 15 ± 5 nm). The changed height value due to the single molecular interaction between integrin α v β 3 and vitronectin was approximately 30 ± 5 nm on Prolinker™ surface as measured with atomic force microscopy tip. Taken together, these results suggest that protein nanoarray on Prolinker™ surface fabricated by well‐controlled DPN process can be used to analyze single molecular interaction of protein.