Premium
Proteomic analysis of a fraction enriched in cell envelope proteins of Acinetobacter baumannii
Author(s) -
Martí Sara,
SánchezCéspedes Javier,
Oliveira Eliandre,
Bellido David,
Giralt Ernest,
Vila Jordi
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500323
Subject(s) - acinetobacter baumannii , microbiology and biotechnology , biology , proteomics , ribosomal protein , shotgun proteomics , proteome , membrane protein , cell envelope , bacterial outer membrane , gene , biochemistry , bacteria , rna , genetics , ribosome , escherichia coli , pseudomonas aeruginosa , membrane
Acinetobacter baumannii is a multiresistant opportunistic nosocomial pathogen. A protein fraction was purified and analyzed by 2‐DE. Twenty‐nine major protein spots were selected for protein identification using trypsin digestion and MS analysis. As the A. baumannii genome has not yet been described, protein identification was performed by homology with other Acinetobacter species in the NCBi database. We identified ribosomal proteins, chaperones, elongation factors and outer membrane proteins (Omp), such as OmpA and the 33–36‐kDa OMP. Proteomic analysis of A. baumannii provides a platform for further studies in antimicrobial resistance.