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Searching for markers of Creutzfeldt‐Jakob disease in cerebrospinal fluid by two‐dimensional mapping
Author(s) -
Piubelli Chiara,
Fiorini Michele,
Zanusso Gianluigi,
Milli Alberto,
Fasoli Elisa,
Monaco Salvatore,
Righetti Pier Giorgio
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500294
Subject(s) - cerebrospinal fluid , cystatin c , transferrin , blot , albumin , microbiology and biotechnology , biology , antibody , proteomics , pathology , peptide mapping , chemistry , gene , medicine , immunology , biochemistry , peptide sequence , renal function
Differential proteomic analysis has been performed on the cerebrospinal fluid (CSF) of six healthy and six patients suffering form sporadic Creutzfeldt‐Jakob disease (sCJD), age‐ and sex‐matched, after immuno‐subtraction of albumin and immunoglobulins. These maps have revealed 28 polypeptide chains differentially modulated in the sCJD samples, of which 10 appeared to be up‐regulated, the remaining 18 being down‐regulated. Among those, 13 could be identified upon digestion and MALDI‐TOF, MS analysis. In addition, the strong modulation of cystatin C was also confirmed by immunoblot analysis and the highly altered level of the 14‐3‐3 proteins that escaped detection by 2‐D mapping, could be assessed by Western blots and immuno‐detection of monomeric and homo‐ and hetero‐dimeric 14‐3‐3 isotypes. In search for a panel of potential markers for sCJD, we highlight cystatin C, 14‐3‐3 proteins, transferrin, ubiquitin, Apoliprotein J and perhaps some of the still unidentified, but strongly modulated polypeptide chains detected in the differential map.