Premium
Potential biomarkers for ischemic heart damage identified in mitochondrial proteins by comparative proteomics
Author(s) -
Kim Nari,
Lee Youngsuk,
Kim Hyungkyu,
Joo Hyun,
Youm Jae Boum,
Park Won Sun,
Warda Mohamad,
Cuong Dang Van,
Han Jin
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500291
Subject(s) - nadh dehydrogenase , malate dehydrogenase , proteomics , pyruvate dehydrogenase complex , blot , prohibitin , biochemistry , citric acid cycle , biology , dehydrogenase , mitochondrion , respiratory chain , microbiology and biotechnology , enzyme , mitochondrial dna , gene
We used proteomics to detect regional differences in protein expression levels from mitochondrial fractions of control, ischemia–reperfusion (IR), and ischemic preconditioned (IPC) rabbit hearts. Using 2‐DE, we identified 25 mitochondrial proteins that were differentially expressed in the IR heart compared with the control and IPC hearts. For three of the spots, the expression patterns were confirmed by Western blotting analysis. These proteins included 3‐hydroxybutyrate dehydrogenase, prohibitin, 2‐oxoglutarate dehydrogenase, adenosine triphosphate synthases, the reduced form of nicotinamide adenine dinucleotide (NADH) oxidoreductase, translation elongation factor, actin alpha, malate dehydrogenase, NADH dehydrogenase, pyruvate dehydrogenase and the voltage‐dependent anion channel. Interestingly, most of these proteins are associated with the mitochondrial respiratory chain and energy metabolism. The successful use of multiple techniques, including 2‐DE, MALDI‐TOF‐MS and Western blotting analysis demonstrates that proteomic analysis provides appropriate means for identifying cardiac markers for detection of ischemia‐induced cardiac injury.