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Proteomic analysis of membrane microdomains derived from both failing and non‐failing human hearts
Author(s) -
Banfi Cristina,
Brioschi Maura,
Wait Robin,
Begum Shajna,
Gianazza Elisabetta,
Fratto Pasquale,
Polvani Gianluca,
Vitali Ettore,
Parolari Alessandro,
Mussoni Luciana,
Tremoli Elena
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500278
Subject(s) - lipid microdomain , caveolae , microbiology and biotechnology , lipid raft , signal transduction , biology , membrane protein , receptor , biochemistry , membrane
Abstract Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such as anchorage of receptors, trafficking of cholesterol, and regulation of permeability. However, an extensive understanding of their protein composition in human heart, both in failing and non‐failing conditions, is not yet available. Membrane microdomains were isolated from left ventricular tissue of both failing ( n = 15) and non‐failing ( n = 15) human hearts. Protein composition and differential protein expression was explored by comparing series of 2‐D maps and subsequent identification by LC‐MS/MS analysis. Data indicated that heart membrane microdomains are enriched in chaperones, cytoskeletal‐associated proteins, enzymes and protein involved in signal transduction pathway. In addition, differential protein expression profile revealed that 30 proteins were specifically up‐ or down‐regulated in human heart failure membrane microdomains. This study resulted in the identification of human heart membrane microdomain protein composition, which was not previously available. Moreover, it allowed the identification of multiple proteins whose expression is altered in heart failure, thus opening new perspectives to determine which role they may play in this disease.