Premium
Proteome analysis of hepatocellular carcinoma by laser capture microdissection
Author(s) -
Ai Jianhua,
Tan Yexiong,
Ying Wantao,
Hong Yi,
Liu Shuqing,
Wu Mengchao,
Qian Xiaohong,
Wang Hongyang
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500257
Subject(s) - laser capture microdissection , proteome , hepatocellular carcinoma , microdissection , proteomics , computational biology , carcinoma , biology , bioinformatics , cancer research , biochemistry , genetics , gene , gene expression
Hepatocellular carcinoma (HCC) is one of the most frequent visceral neoplasia worldwide and is a multifactorial and multistage pathogenesis that finally leads to the deregulation of cell homeostasis. Laser capture microdissection (LCM) may allow a more ready identification of differences in protein expression in selected cell types or areas of tissue, and microscopic regions as small as 3–5 μm in diameter can be sampled. Here we applied the LCM to the proteomic study of hepatitis B‐related HCC and surrounding non‐tumor tissues. Proteome alterations were observed using 2‐DE and ESI‐MS/MS, and alterations in the proteome were examined. Twenty protein spots were selected, of which 11 proteins were significantly altered in the HCC compared with the surrounding non‐tumor tissues. Of the proteins that were selected, peroxiredoxin 2, apolipoprotein A‐I precursor, 3‐hydroxyacyl‐CoA dehydrogenase type II, and 14.5‐kDa translational inhibitor protein appear to be novel candidates as useful hepatitis B‐related HCC markers. This study indicates that LCM is a useful technological method in the proteomic study of cancer tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC associated with hepatitis B virus infection.