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A modified tandem affinity purification strategy identifies cofactors of the Drosophila nuclear receptor dHNF4
Author(s) -
Yang Ping,
Sampson Heidi M.,
Krause Henry M.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500230
Subject(s) - tandem affinity purification , cofactor , biology , biochemistry , function (biology) , nuclear receptor , flag tag , dna binding protein , nuclear protein , gene , microbiology and biotechnology , affinity chromatography , recombinant dna , transcription factor , fusion protein , enzyme
With the completion of numerous genome projects, new high‐throughput methods are required to ascribe gene function and interactions. A method proven successful in yeast for protein interaction studies is tandem affinity purification (TAP) of native protein complexes followed by MS. Here, we show that TAP, using Protein A and CBP tags, is not generally suitable for the purification and identification of proteins from tissues. A head‐to‐head comparison of tags shows that two others, FLAG and His, provide protein yields from Drosophila tissues that are an order of magnitude higher than Protein A and CBP. FLAG‐His purification worked sufficiently well so that two cofactors of the Drosophila nuclear receptor protein dHNF4 could be purified from whole animals. These proteins, Hsc70 and Hsp83, are important chaperones and cofactors of other nuclear receptor proteins. However, this is the first time that they have been shown to interact with a non‐steroid binding nuclear receptor. We show that the two proteins increase the ability of dHNF4 to bind DNA in vitro and to function in vivo . The tags and approaches developed here will help facilitate the routine purification of proteins from complex cells, tissues and whole organisms.