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SELDI‐TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions
Author(s) -
Traum Avram Z.,
Wells Meghan P.,
Aivado Manuel,
Libermann Towia A.,
Ramoni Marco F.,
Schachter Asher D.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500174
Subject(s) - urine , concordance , chromatography , liquid nitrogen , biomarker discovery , medicine , chemistry , proteomics , biochemistry , organic chemistry , gene
Proteomic profiling with SELDI‐TOF MS has facilitated the discovery of disease‐specific protein profiles. However, multicenter studies are often hindered by the logistics required for prompt deep‐freezing of samples in liquid nitrogen or dry ice within the clinic setting prior to shipping. We report high concordance between MS profiles within sets of quadruplicate split urine and serum samples deep‐frozen at 0, 2, 6, and 24 h after sample collection. Gage R&R results confirm that deep‐freezing times are not a statistically significant source of SELDI‐TOF MS variability for either blood or urine.