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Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish
Author(s) -
Zupanc Marianne M.,
Wellbrock Ursula M.,
Zupanc Günther K. H.
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500167
Subject(s) - fish <actinopterygii> , regeneration (biology) , proteome , cerebellum , proteomics , biology , computational biology , microbiology and biotechnology , bioinformatics , fishery , biochemistry , neuroscience , gene
In contrast to mammals, adult teleost fish exhibit an enormous potential to regenerate neuronal tissue after injuries to the CNS. By combining a well‐defined cerebellar lesion paradigm with differential proteome analysis at a post‐lesion survival time of 3 days, we screened for protein candidates involved in repair of the fish brain. Out of nearly 900 protein spots detected on 2‐D gels, spot intensity was significantly increased at least twofold in 30 spots and decreased to at least half the intensity of control tissue in 23 spots. The proteins associated with 24 of the spots were identified by PMF and MS/MS fragmentation. The cellular localization and the spatiotemporal patterns of two of these proteins, beta‐actin and beta‐tubulin, were further characterized through immunohistochemistry. Comparison of the observed changes in protein abundance with the previously characterized events underlying regeneration of the cerebellum suggests that the proteins identified are especially involved in cellular proliferation and survival, as well as axonal sprouting.