DIGE compatible labelling of surface proteins on vital cells   in vitro   and   in vivo | Zendy

Mayrhofer Corina | Zendy; Krieger Sigurd | Zendy; Allmaier Günter | Zendy; Kerjaschki Dontscho | Zendy

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DIGE compatible labelling of surface proteins on vital cells in vitro and in vivo

Author(s) -

Mayrhofer Corina,

Krieger Sigurd,

Allmaier Günter,

Kerjaschki Dontscho

Publication year - 2006

Publication title -

proteomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.26

H-Index - 167

eISSN - 1615-9861

pISSN - 1615-9853

DOI - 10.1002/pmic.200500104

Subject(s) - proteome , labelling , in vitro , fluorophore , in vivo , proteomics , cell , microbiology and biotechnology , cell culture , biology , human proteome project , biochemistry , chemistry , fluorescence , computational biology , genetics , gene , physics , quantum mechanics

Efficient methods for profiling of the cell surface proteome are desirable to get a deeper insight in basic biological processes, to localise proteins and to uncover proteins differentially expressed in diseases. Here we present a strategy to target cell surface exposed proteins via fluorescence labelling using CyDye DIGE fluors. This method has been applied to human cell lines in vitro as well as to a complex biological system in vivo . It allows detection of fluorophore‐tagged cell surface proteins and visualisation of the accessible proteome within a single 2‐D gel, simplifying subsequent UV MALDI‐MS analysis.

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