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1‐DE MS and 2‐D LC‐MS analysis of the mouse bronchoalveolar lavage proteome
Author(s) -
Guo Yurong,
Ma ShwuFan,
Grigoryev Dmitry,
Van Eyk Jennifer,
Garcia Joe G. N.
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500052
Subject(s) - proteome , bronchoalveolar lavage , proteomics , peptide , chemistry , chromatography , microbiology and biotechnology , biology , computational biology , biochemistry , lung , medicine , gene
Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2‐D LC microESI MS/MS and 1‐DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.