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Proteomic analysis of rat pheochromocytoma PC12 cells
Author(s) -
Yang Wei,
Liu Peng,
Liu Yashu,
Wang Qingsong,
Tong Yuanpeng,
Ji Jianguo
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500036
Subject(s) - proteome , proteomics , cell culture , chemistry , microbiology and biotechnology , computational biology , gene , biology , database , biochemistry , genetics , computer science
PC12 cell line is well documented and widely applied as many kinds of models in neurobiological and neurochemical studies. Yet a thorough proteomic analysis has not been performed so far. Here we report the construction of a large‐scale 2‐D protein database for PC12 cells. The proteins extracted from PC12 cells were separated by 2‐DE and identified by MALDI‐TOF/TOF MS. A total of 1080 protein spots, excised from three different 2‐D gels, were identified with high confidence. These proteins represent 474 different gene products, mainly binding proteins and enzymes. Three hundred and seven identified protein spots were located in the low‐molecular weight region below 20 kDa. This database today represents one of the largest 2‐D databases for higher eukaryotic cell proteomes and for low‐molecular weight proteins. In addition, fragment ion spectra obtained by TOF/TOF confirmed that calcylin in PC12 cells was N‐acetylated. The database of PC12 proteome is expected to be a powerful tool for neuroscientists.